The following 28 characters are playable in Toshiko Crash-Up, 10 being playable at the start, while 18 are unlockable. The line-up includes mostly 1st characters, with two 3rd party characters. The characters are listed in the order they appear in the Character Select screen in the game.
|Duke of Zorn|
|Jack and Spooks|
There are several characters who appear as alternate costumes for already existing characters including: